Cytogenetics Transcription Eukaryotes Chromatin Remodelling Online Exam Quiz
Cytogenetics Transcription Eukaryotes Chromatin Remodelling GK Quiz. Question and Answers related to Cytogenetics Transcription Eukaryotes Chromatin Remodelling. MCQ (Multiple Choice Questions with answers about Cytogenetics Transcription Eukaryotes Chromatin Remodelling
In an experiment you perform the following- you attach a flag epitope to HDAC, you over express a mutated Mad1-pro gene within the experimental cell, you immunoprecipitate the protein complex using flag epitope and run that on a non-denaturing SDS PAGE and western blot. Now you add antibodies for HDAC, MAD1 and SIN3A stripping after each addition. What will you observe?
Options
A : Bands at same position every time
B : 3 bands at different positions one at a time
C : Bands at same position on 2 occasions
D : No band
In which of the following chromosome compaction is absent?
Options
A : Cellulomonas flavigena
B : Allium sativum
C : Panthera tigris
D : Saccharomyces cerevisiae
In a substrate X catabolic gene promoter you replace the normal histones by your experimental histones – ones with arginine side chains in place of histidine. What will be the effect on the concentration of substrate X against the control?
Options
A : Concentration will increase
B : Concentration will decrease
C : Concentration will remain constant
D : Concentration will fluctuate and no definite result will be obtained
Which histone(s) are acted upon by HAT?
Options
A : H1, H2
B : H3
C : H2A, H2B
D : H3, H4
You take a crude cell lysate and treat it with DNase. Now wash it and add protease. Use specific restriction endonuclease to get your desired fragment and run it on a gel. You see a single band. Which of the following will be true about the fragment?
Options
A : It is actively transcribed
B : Only part of it is transcribed
C : No part of it transcribed
D : Undefined transcriptional nature
Which of the following is not a common mode of histone modification in eukaryotes?
Options
A : Methylation
B : Phosphortlation
C : Sulphonation
D : Ubiquitinylation
Which of the following association acts as transcriptional activator?
Options
A : Myc- Max
B : Mad- Max
C : Map- Mad
D : MapK- Myc
Histones are __________
Options
A : Neutral
B : Positively charged
C : Negatively charged
D : Neutral with positive and negative domains
In presence of Thyroid hormone the receptor TR binds to ____________
Options
A : RXR directly
B : mRPD3 directly
C : NcoR directly
D : mRPD3 indirectly
Which of the following will increase transcription?
Options
A : Shielding positive charge of DNA
B : Shielding positive charge of histones
C : Shielding promoter for polymerase binding
D : Shielding termination region
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