Cytogenetics Transcription Prokaryotes Elongation Online Exam Quiz
Cytogenetics Transcription Prokaryotes Elongation GK Quiz. Question and Answers related to Cytogenetics Transcription Prokaryotes Elongation. MCQ (Multiple Choice Questions with answers about Cytogenetics Transcription Prokaryotes Elongation
What is the 1st nucleotide of a completely synthesized transcript?
Options
A : A
B : G
C : C
D : T
You add labeled Uridine analogue to the mixture of DNA and RNA pol in vitro. What are you trying to determine?
Options
A : Catalytic subunit of RNA pol
B : Processivity of RNA pol
C : Presence of DNA – RNA hybrid
D : Length of DNA already transcribed
Melting of DNA would lead to _________________
Options
A : Increase in UV absorption
B : Increase in Fluorescence
C : Decrease in UV absorption
D : Decrease in fluorescence
Capping of RNA is necessary as_______
Options
A : It helps us distinguish 5’ from 3’ end
B : It has a rolling action and condenses the transcript as it is produced
C : To protect the transcript from exonuclease
D : To prevent the transcript from sticking to DNA
You design a reconstructive RNA polymerase where you take the beta and alpha subunit from a rifampicin resistant strain and beta prime and sigma subunit from rifampicin sensitive strain. You check the rate of transcription by this RNA polymerase after the addition of the antibiotic. What will be your observation?
Options
A : No transcription
B : Reduced transcription
C : Normal transcription
D : Improved transcription
Which of these is not a part of RNA polymerase elongation machinery?
Options
A : NTP entry channel
B : RNA entry channel
C : DNA entry channel
D : Clamp
In a stretch of DNA being transcribed, the region forward to the Elongation is __________
Options
A : Positively supercoiled
B : Negatively supercoiled
C : Uncoiled
D : Forms a secondary structure
While studying the transcripts in vitro you observe that other than the longer transcripts there are some short stretches of RNA oligomer produced as well. You repeat the experiment several times but these oligomers seem to persist. What is the possible explanation?
Options
A : Procedural error
B : They are the un-ligated oligomers of lagging strand
C : Experimental artifact
D : Normal occurrence
Which antibiotic inhibits transcription elongation?
Options
A : Rifampicin
B : Streptolydigin
C : Penicillin
D : Tetracyclin
Processivity of RNA polymerase is ________
Options
A : Very high
B : High
C : Low
D : Very low
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