Cytogenetics Restriction Endonuclease Online Exam Quiz
Cytogenetics Restriction Endonuclease GK Quiz. Question and Answers related to Cytogenetics Restriction Endonuclease. MCQ (Multiple Choice Questions with answers about Cytogenetics Restriction Endonuclease
Methylated DNA doesn’t form kink as________________
Options
A : As they don’t fit in the active site
B : The enzyme recognized methylated A as different base than non-methylated A
C : They are less flexible so no kink is formed
D : They don’t interact with the side chains in an active site
Which of the sequence will Dcm methylate?
Options
A : GATC
B : GAATC
C : CCGTT
D : CCAGG
Which of the following is not true about type II restriction endonuclease?
Options
A : Here Clevage and Methylation are by different enzymes
B : In this case the recognition sequence is asymmetric
C : It cleaves within the recognition sequence
D : It can’t cleave at methylated sequences
In EcoRV the enzyme is a ____________
Options
A : Homo dimer
B : Hetero dimer
C : Trimer
D : Hetero tetramer
When the DNA enzyme interacts with the cognate DNA, there is _______________
Options
A : Increase in free energy
B : Reduction in free energy
C : Breakdown of the complex
D : Repulsion
Which of the following is not true for type 1 restriction endonuclease?
Options
A : It is a single enzyme with 3 subunits
B : Using it is an energy requiring process
C : Methylation and cleavage occurs in the same sequence
D : The cleavage is up to 1000bp away
Restriction enzymes cleave the bond by which type of reaction?
Options
A : SN1
B : SN2
C : SE1
D : SE2
Which of these restriction enzymes generate 3’ overhang?
Options
A : Sph1
B : EcoR1
C : Sma1
D : Xma1
Which of the following enzyme air is a neoisoschizomer?
Options
A : SphI and BbuI
B : Not1 and HindIII
C : SmaI and XmaI
D : Pst1 and Sma1
Restriction endonucleases can recognize___________
Options
A : Tandem repeats
B : Pallindromic sequences
C : GATC
D : No sequence specificity
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