Biochemistry Working Proteins Online Exam Quiz
Biochemistry Working Proteins GK Quiz. Question and Answers related to Biochemistry Working Proteins. MCQ (Multiple Choice Questions with answers about Biochemistry Working Proteins
The pattern on paper in chromatography is called __________
Options
A : Chroming
B : Chroma
C : Chromatograph
D : Chromatogram
Which of the following statements is true about size-exclusion chromatography?
Options
A : During the separation of a mixture of proteins, protein with smallest molecular weight is eluted first
B : During the separation of a mixture of proteins, protein with largest molecular weight is eluted first
C : During the separation of a mixture of proteins, protein with largest molecular weight is eluted last
D : During the separation of a mixture of proteins, protein with largest molecular weight flow around the beads
Which of the following statements is true about affinity chromatography?
Options
A : During the separation of a mixture of proteins, the protein which does not bind to ligand is eluted first
B : During the separation of a mixture of proteins, the protein which does not bind to ligand is eluted last
C : During the separation of a mixture of proteins, the protein which binds to ligand is eluted first
D : Unwanted proteins are eluted by ligand solution
Which of the following statements is true about ion-exchange chromatography?
Options
A : It separates proteins according to their size
B : The column matrix with bound anionic groups is called cationic exchanger
C : The column matrix with bound anionic groups is called anionic exchanger
D : The column matrix with bound cationic groups is called cationic exchanger
The salt which produces salting out effect during extraction of proteins is?
Options
A : NH4 SO4
B : (NH4)2 SO4
C : (NH4)3 SO4
D : NaCl
Mobile phase can be ___________
Options
A : Only solid
B : Only gas
C : Solid or liquid
D : Liquid or gas
Which of the following statements about column chromatography is correct?
Options
A : Resolution increases as the length of the column increases
B : Mobile phase is a porous solid material with appropriate chemical properties held in the column
C : Stationary phase is a buffered solution that percolates through mobile phase
D : Large proteins emerge from the column sooner than small ones
Which of the following statements is true about SDS polyacrylamide chromatography?
Options
A : SDS polyacrylamide gel electrophoresis separates proteins on the basis of size
B : SDS polyacrylamide gel electrophoresis separates proteins on the basis of charge
C : SDS binds to proteins non-covalently with a stoichiometry of around one SDS molecule per three amino acids
D : SDS binds to proteins non-covalently with a stoichiometry of around one SDS molecule per one amino acid
Which of the following statements is true about two-dimensional electrophoresis?
Options
A : Separates proteins of identical molecular weight, same pI but different charge
B : Separates proteins of different molecular weight and different pI
C : Separates proteins of identical molecular weight that differ in pI
D : Isoelectric focusing is also termed as two-dimensional electrophoresis
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