Question: Which of the following statements is true about affinity chromatography?

Options

A : During the separation of a mixture of proteins, the protein which does not bind to ligand is eluted first

B : During the separation of a mixture of proteins, the protein which does not bind to ligand is eluted last

C : During the separation of a mixture of proteins, the protein which binds to ligand is eluted first

D : Unwanted proteins are eluted by ligand solution

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