Biochemistry Dna Cloning Basics 1 Online Exam Quiz

Biochemistry Dna Cloning Basics 1 GK Quiz. Question and Answers related to Biochemistry Dna Cloning Basics 1. MCQ (Multiple Choice Questions with answers about Biochemistry Dna Cloning Basics 1

Generally a plasmid vector contains how many elements?

Options

A : 1

B : 2

C : 3

D : 4

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What is a DNA library?

Options

A : A DNA fragment inserted into a vector

B : A general collection of all genes sequenced thus far

C : All DNA fragments identified with a probe

D : A collection of DNA fragments that make up the entire genome of a particular organism

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Which of the following enzymes’ combined action leads to the generation of sticky ends in the plasmid vector?

Options

A : Alkaline phosphatase and terminal transferase

B : Exonuclease III and alkaline phosphatase

C : Bacteriophage lambda exonuclease and terminal transferase

D : Exonuclease III and terminal transferase

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The extra chromosomal, self-replicating, closed, double stranded and circular DNA molecule is generally termed as __________

Options

A : Chromosome

B : Plasmid

C : Genomic DNA

D : Bacteriophage

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In which stage of genetic engineering a probe is used?

Options

A : Cleaving DNA

B : Recombining DNA

C : Cloning

D : Screening

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Which of the following enzyme is required for end to end joining of DNA?

Options

A : DNA ligase

B : Restriction endonuclease

C : RNA polymerase

D : DNA polymerase

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E.cor1 is a __________

Options

A : DNA ligase enzyme

B : Restriction endonuclease

C : A vector used for insulin synthesis

D : A plasmid used as a vector

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Which of the following does not act as a restriction enzyme?

Options

A : EcorI

B : BamHI

C : HindIII

D : polydeoxyribonucleotide synthase

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To make the recombinant plasmid permeable to DNA molecules, which of the chemicals is added?

Options

A : MgCl2

B : CaCl2

C : NaCl

D : HCl

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Repressor molecules bind to __________

Options

A : Operator

B : Promoter

C : Enhancer

D : Hormone response element

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