Question: Which of the following cannot be related to multiple sequence alignment?
Options
A : Many conserved and functionally critical amino acid residues can be identified in a protein multiple alignment
B : Multiple sequence alignment is also an essential prerequisite to carrying out phylogenetic analysis of sequence families and prediction of protein secondary and tertiary structures
C : Multiple sequence alignment also has applications in designing degenerate polymerase chain reaction (PCR) primers based on multiple related sequences
D : This method does not contribute much to degenerate polymerase chain reaction (PCR) primers creation
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